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High quality papain enzyme,CAS:9001-73-4
High quality papain enzyme,CAS:9001-73-4Produced under GMP,ISO certificateUSP/BP standard
Description
papain enzyme/9001-73-4
1 Active Unit: 200000iu-3000000iu/gm
2 2 White ~light yellow powder
General description
The mechanism by which it breaks peptide bonds involves deprotonation of Cys-25 by His-159. Asparagine-175 helps to orient the imidazole ring of His-159 to allow this deprotonation to take place. Cys-25 then performs a nucleophilic attack on the carbonyl carbon of a peptide backbone. This frees the amino terminal of the peptide, and forms a covalent acyl-enzyme intermediate. The enzyme is then deacylated by a water molecule, and releases the carboxy terminal portion of the peptide. In immunology, papain is known to cleave the Fc (crystallisable) portion of immunoglobulins (antibodies) from the Fab (antigen-binding) portion.Papain is relatively heat resistant enzyme
Function and Application
1 Its utility is in breaking down tough meat fibers
2 dissociate cells in the first step of cell culture preparations
3 Papain can also be found as an ingredient in some toothpastes or mints as teeth-whitener.
4 it is the main ingredient of Papacarie
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High quality papain enzyme,CAS:9001-73-4